User

Effect of Antihipoglycemic Sechium edule Jacq. Swartz. Etanol Extract on Histopathologic Changes in Hyperglycemic Mus musculus L.

Jekson Martiar Siahaan

Abstract

Background: Streptozotocin as diabetogenic can damage the pancreatic β cells of animals tried through the oxidative stress process to increase blood sugar levels. Giving ethanolic extract of squash fruit has hypoglycemia effect because it contains flavonoids that act as antioxidants and antihiperglichemia. This study aimed is analyze the effect of ethanol extract of squash fruit to decrease blood sugar level and the change of pancreatic β cell diameter.

Subjects and Method: This study was an experimental study with post-randomized controlled group design, using male white mice (Mus musculus L.) DD Webster strains randomized into 4 groups: negative control group, positive control group, group with extract ethanol of 100 mg/ kgBB, and a group of ethanol extract of 200 mg/ kgBW of pumpkin.

Results: The results showed a significant reduction in blood sugar levels if compared with the control group. The presence of changes in β pankreas cell diameter on ethanol extract of 100 mg/ kgBB and 200 mg/ kgBB.

Conclusion: The conclusion of this study is the extract of ethanol fruit of 200mg/ kgBB squash significantly reduce blood sugar level of mice, the change of β pankreas cell diameter on ethanol extract of 100mg/ kgBB and 200mg/ kgBB.

Keywords: streptozotocin, antihipoglikemia, flavonoid

Correspondence: Jekson Martiar Siahaan. Physiology Teaching Staff ofMedicine Faculty, Methodist University of Indonesia Medan, Jl. Setia Budi Pasar 2, Medan 20132, Indonesia.

Journal of Medicine (2017), 2(2): 86-93
https://doi.org/10.26911/theijmed.2017.02.02.02

Full Text:

PDF

References

Akbarzadeh AD, Norouzian MR, Mehrabi Sh, Jamshidi,Farhangi, Verdi AA, Mofidian SMA, Rad BL (2007). Induction Of DM By Streptozotocin In Rats. Indian Journal of Clinical Biochemistry. 22 (2): 60-64.

Atalay M, Laaksonen DE (2002). DM, oxidative stress and physical exercise. Journal of Sports Science and Medicine. 1: 1-14.

Brahmachari, Goutam (2011). Bioflavono-ids with promising antidiabetic potentials: A critical survey. Study signpost, 6619370:187-212.

Erwin, Etriwati, Rusli (2012). Mencit (Mus musculus) galur balbc yang diinduk ikan streptozotosin berulang sebagai hewan model DM. Jurnal Kedokteran Hewan 6(1).

Gethaa, Sahgal, Ramanathan S (2010). Brine Shrimp Lethality and Acute Oral Toxicity Studies on Swetwnia Mahagoni (Lin) Jacq. Seed Methanolic Extract. Pharmacognosy Study. 2(4): 215-220.

Moussa SA (2008). Oxidative stress in diabetes mellitus. Rom J Biophys 18(3): 225–36.

Nugroho AE (2006). Animal models of diabetes mellitus: Pathology and mechanism of some diabetogenics. Biodiversitas, J Biol Divers 7(4):378–82.

Park, Han MH, Ji Sook (2012). Hypoglycemic Effect of Padina arborescens Extract in Streptozotocin induced Diabetic Mice, Prev Nutr Food Sci. 17: 239 – 244.

Raza, Haider, John A (2012). Streptozotocin Induced Cytotoxicity, Oxidative Stress and Mitochondrial Dysfunction in Human Hepatoma HepG2 Cells. Int. J. Mol. Sci. 13, 5751-5767; doi:10. 3390/ijms 1305-5751.

Redha A (2010). Flavonoid: Struktur, Sifat Antioksidatif Dan Peranannya dalam Sistem Biologis. Jurnal Belian. 9(2).

Ridwan A, Astrian RT, Barlian A (2012). Pengukuran Efek Antidiabetes Polifenol (Polyphenon 60) Berdasarkan Kadar Glukosa Darah dan Histologi Pankreas Mencit (Mus musculus L.) S.W. Jantan yang Dikondisikan DM. Jurnal Matematika & Sains. 17(2).

Robertson RP, Harmon JS (2007). Pancreatic islet β-cell and oxidative stress: The importance of glutathione peroxidase. FEBS Lett 581(19): 3743–8.

Rohyami Y (2008). Penentuan Kandungan Flavonoid dari Ekstrak Metanol Daging Buah Mahkota Dewa (phaleria macrocarpa Scheff Boerl). Logika. 5(1).

Maity S, Firdous SM, Debnath R (2013). Evaluation of antidiabetic activity of ethanolic extract of Sechium edule fruits in alloxan induced diabetic rats. World J Pharm Pharm Sci 2(5): 3612–21.

Marliana SD, Suryanti V (2005). Skrining fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq.Swartz.) dalam Ekstrak Etanol. Biofarmasi 3(1):26–31.

Setiawan B, Suhartono E. (2005). Stres Oksidatif dan Peran Antioksidan pada DM. Majalah Kedokteran Indonesia. 55(2). Available from http://mki.idionline.org/index.php?uPage=mki.mki_dl&smod=mki&sp=public&key=MTItMTQ.

Suryani N, Tinny EH, Aulanni’am (2013). Pengaruh Ekstrak Metanol Biji Mahoni terhadp Peningkatn Kadr Insulin, Penurunan Ekspresi TNF-α dan Perbaikan Jaringan Pankreas Tikus DM. Jurnal Kedokteran Brawijaya. 27(3)

Piparo Elo, Scheib H, Frei N, Williamson G, Grigorov M, Chou CJ (2008). Flavonoids for controlling starch digestion: structural requirements for inhibiting human ramylase 3555–61.

Shradha B, Sisodia SS (2010). DM, Dyslipidemia, Antioxidant And Status Of Oxidative Stress. International Journal of Study in Ayurveda & Pharmacy, 1(1).

Sheikhpour R (2013). Diabetes and oxidative stress : The mechanism and action. Iran J diabtes Obes 5(1).

Siciliano T, De Tommasi N, Morelli I, Braca A (2004). Study of flavonoids of Sechium edule (Jacq) Swartz (Curcubitaceae) different edible organs by liquid chromatography photodiode array mass spectrometry. Journal of Agricultural and Food Chemistry: 6510–6515.

Srinivasan K, Ramarao P (2007). Animal models in type 2 diabetes study: an overview. Indian J Med Res 125(3): 451–72.

SuarsanaIN, Priosoeryanto BP, Bintang M, Wresdiyati T (2010). Profil Glukosa Darah dan Ultrastruktur Sel Β Pankreas Tikus yangDiinduksi Senyawa Aloksan. JITV, 15(2): 118-123.

Refbacks

  • There are currently no refbacks.