Toxicity Test of Sentul Fruit (Sandoricum koetjape) Peel Extract on Mice
Abstract
Background: Sentul fruit peel extract has an antibacterial activity since it contains saponin, flavonoid, phenol, and tannin. Hence, it can be utilized in oral infection treatment. The safety level of herbal medicines needs to be studied by using a toxicity test. The study aims to discover the toxicity of Sentul fruit peel extract.
Subjects and Method: The study was conducted in the laboratory of the Faculty of Food Technology of Warmadewa University and the laboratory of the Faculty of Medicine and Health Sciences of Warmadewa University. The independent variables of the study were the various doses of Sentul peel extract administered orally. The dependent variable was an acute toxicity test. The toxicity test in mice used an experimental study design in the laboratory that was observed qualitatively and quantitatively. The animals used were 25 male white mice (Mus musculus) and were divided into 5 groups. Data were collected based on the results of examinations from the laboratory and subsequently analyzed qualitatively and descriptively.
Results: Based on the results of the LD50 Toxicity Test on Mice, Sentul peel extract samples did not cause toxic symptoms in the form of decreased heart activity, convulsions, decreased movement activity, and slow breathing. The administration of Sentul peel extract was categorized as non-toxic because mice do not experience abnormal symptoms and there was no death after the administration of the extract using the oral gavage method at all doses for 14 days, thus Sentul peel extract was safe to use as a medicinal ingredient.
Conclusion: Sentul peel extract is categorized as non-toxic because mice do not experience abnormal symptoms and no death occurs.
Keywords: Sentul peel, extraction, oral infection, mice, toxicity
Correspondence: I Nyoman Wirata, Health Polytechnic of the Ministry of Health Denpasar. Jl. Sanitasi No.1, Sidakarya, South Denpasar, Denpasar City, Bali 80224. Email: wiratainyoman@gmail.com. Mobile: 08123649998.
Indonesian Journal of Medicine (2023), 08(01): 114-120
https://doi.org/10.26911/theijmed.2023.08.01.11
References
Ambarwati (2007). Kajian actinomycetes yang berpotensi menghasilkan anti-biotika dari rhizosfer putri malu (Mimosa pudica L.) dan kucing-kucingan (Acalypha indica L.). Jurnal Sains dan Teknologi. 8(1): 1-14.
Anastasia N (2010). Uji aktivitas anti bakteri senyawa alfa mangostin kulit buah manggis (Garcinia mangostana l.) terhadap propionibacterium acne dan staphylococcus aureus multires-isten. Thesis. Faculty of Pharmacy: Universitas Muhammadiyah Surakarta. http:-//eprints.ums.ac.id/id/eprint/10089.
Aria WU, Efdi M, Santoni A (2013). Isolasi senyawa triterpenoid dari fraksi aktif kulit batang kecapi (Sandoricum koetjape merr) dan uji bioaktifitas brines-hrimps lethality bioassa. Jurnal Kimia Unand. 2(1).
BPOM RI (2014). Pedoman uji toksisitas non klinik secara in vivo. Jakarta.
Caninsti R (2007). Gambaran kecemasan dan depresi pada penderita gagal gin-jal kronis yang menjalani terapi hemodialisa). Depok: Pascasarjana Fakultas Psikologi UI.
Dipasquale LC, Hayes AW (2001). Acute toxicity and eye irritancy, dalam hayes, a.w. (ed.), principles and methods of toxicology, 4th ed.
Gani S (1995). Farmakologi dan terapi, Edisi 4. Jakarta: Bagian Farmakologi, Fakultas Kedokteran Universitas Indonesia. 1995: 7637.
Katzung BG (2002). Farmakologi dasar dan klinik. Jakarta Salemba Medika. Terjemahan dari: Basic dan Clinical Pharmacology Ed ke-8.
Marlinda M, Sangi MS, Wuntu AD (2012). Analisis senyawa metabolit sekunder dan uji toksisitas ekstrak etanol biji buah alpukat (Persea americana Mill). Jurnal MIPA UNSRAT.
Meles KD (2010). Peran uji praklinik dalam bidang farmakologi. Pusat penerbitan dan percetakan Unair (AUP). Perpustakaan Universitas Airlangga: Surabaya.
Nonci FY, Rusdi M, Mohan IZFL (2014). Uji toksisitas akut ekstrak etanol klika jambu mede (Anacardium occidentale L.) pada mencit jantan (Mus musculus). JF FIK UINAM. 2(2) :62-68.
OECD (2001). Guidelines for testing of chemicals test no. 420: acute oral toxicity: fixed-dose procedure. 4-8.
Priyanto (2010). Toksikologi Ed: 2. Depok: leskonfi lembaga studi dan konsultasi farmakologi.
Rosdiana I, Yetty K, Sabri L (2014). Kece-masan dan lamanya waktu menjalani hemodialisis berhubungan dengan kejadian insomnia pada pasien gagal ginjal kronik. Jurnal Keperawatan In-donesia. 17(2): 39-47.
Sabry AA, Abo-Zenah H, Wafa E, Mahmoud K, El-Dahshan K, Hassan A, Abbas TM (2010). Sleep disorders in hemodialysis patients. Saudi J Kidney Dis Transpl. 21: 300-5.
Silaban, Lowysa W (2009). Skrining fitokimia dan uji aktivitas anti bakteri dari kulit buah sentul (Sandoricum koetjae (burm. f.) merr) terhadap beberapa bakteri secara in vitro. Skripsi. Medan: USU.
Smeltzer SC, Bare BG, Hinkle JL, Cheever KH (2008). Textbook of medical surgical nursing (11th Ed.). Philadelphia: Lippincott William and Wilkins.
Swantara MD, Ciawi Y (2009). Identifikasi senyawa antibakteri pada daun kecapi. Jurnal Kimia. 3(2): 61-68.
Syahrurahman A (2010). Buku ajar mikro biologi Kedokteran. Edisi Revisi.
Tarwoto, Wartonah (2011). Kebutuhan dasar manusia dan proses keperawatan. Jakarta: Salemba Medika.
Warsinah, kusumawanti E, Sunarto (2011). Identidikasi senyawa antifungi dari kulit batang kecapi (Sandoricum koetjape) dan aktivitasnya terhadap candida albicans. majalah obat tradisional. 16(3): 165-173.
Wirata IN, Agung AA, Gede A, Ni W, Nuratni, Ni K (2021). Sentul fruit (Sandoricum koetjape) peel as anti-inflammation for gingivitis after scaling. J health sci med. 4(4): 27-36.
