Effect of Ethanolic Extract of Moringa oleifera, Lam. Roots on Expression of Cyclin D1 in Testicular Tissue of Metabolic Syndrome Induced Wistar Rats
DOI:
https://doi.org/10.26911/theijmed.2023.8.2.562Abstract
Background: Metabolic syndrome (MS) impacts on inhibition process of spermatogenesis by damaging various components of cell cycle proteins. Cyclin D1 protein which plays role in regulating the cell cycle of spermatogenic cells is depleted due to the metabolic syndrome. Variation results of M. oleifera studies on fertility are interesting for further research. This study aimed to determine the effect of ethanolic extract of M. oleifera roots towards expression of testicular cyclin D1 of metabolic syndrome induced Wistar rats.
Subjects and Method: This study was an experimental laboratory. Thirty subjects of male Wistar rats were randomly divided into 5 groups consisted of 6 rats, control group (K1), MS group (K2), and treatment groups (K3, K4, and K5) which were given doses of 150, 250, and 350 mg/kgBW, respectively for 28 days. Induction of MS was achieved by high-fat diet for 28 days and injection of streptozotocin-nicotinamide (STZ-NA) on day 25. Cyclin D1 data was calculated using Intensity Distribution Score (IDS) and analyzed using one-way ANOVA test followed by post hoc multiple comparisons with Tukey HSD. Simple liniar regression test conducted on cyclin D1 data of K2, K3, K4, and K5.
Results: One-way ANOVA test showed that the administration of ethanolic extract M. oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW in 28 days increased cyclin D1 significantly (p= 0.000). There were significant differences between K1 and K2 (p= 0.000), K2 and K3 (p= 0.000), K2 and K4 (p= 0.000), K2 and K5 (P= 0.000). Liniar regression test showed significant (p= 0.000) and moderate effect (R2= 0.65) of doses of ethanolic extract of M. oleifera roots on the expression of cyclin D1.
Conclusion: The administration of ethanolic extract Moringa oleifera Lam. roots with doses of 150, 250, and 350 mg/kgBW for 28 days increased cyclin D1 in testicular rats.
References
Abass O (2015). Compositional investigation of phytochemical and antioxidant properties of various parts of moringa oleifera plant 1. European Journal of Basic and Applid Science. 2(2): 1–10.
Adewoyin M, Ibrahim M, Roszaman R, Isa M, Alewi N, Rafa A, Anuar M (2017). Male Infertility: The Effect of Natural Antioxidants and Phytocompounds on Seminal Oxidative Stress. Diseases. 5(1): 9. https://doi.org/10.3390/diseases5010009
Alkafafy ME, Sayed SM, El‐Shehawi AM, El‐Shazly S, Farouk S, Alotaibi SS, Madkour DA, et al. (2021). Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model. Andrologia. 53(8): 1–12. https://doi.org/10.1111/and.14126
Al-Malki AL, El Rabey HA (2015). The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats. BioMed Research International. 2015: 1–13. https://doi.org/10.1155/2015/381040
Carrera-Chávez JM, Jiménez-Aguilar EE, Acosta-Pérez TP, Núñez-Gastélum JA, Quezada-Casasola A, Escárcega-Ávila AM, et al. (2020). Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen. Journal of Applied Animal Research. 48(1): 114–120. https://doi.org/10.1080/09712119.2020.1741374
Carrier A (2017). Metabolic Syndrome and Oxidative Stress: A Complex Relationship. Antioxidants & Redox Signaling. 26(9): 429–431. https://doi.org/10.1089/ars.2016.6929
Dafaalla MM, Abass S, Abdoun S, Hassan AW, Idris OF (2017). Effect of ethanol extract of Moringa oleifera seeds on fertility hormone and sperm quality of male Albino Rats. International Journal of Multidisciplinary Research and Development. 4(4): 222–226. Retrieved from www.allsubjectjournal.com
El-Wakf AM, El-Habibi ESM, Ali DA, Abd El-Ghany E, Elmougy R (2020). Marjoram and sage oils protect against testicular apoptosis, suppressed Ki-67 expression and cell cycle arrest as a therapy for male infertility in the obese rats. Journal of Food Biochemistry. 44(1): 1–11. https://doi.org/10.1111/jfbc.13080
Etejere EO, Olayinka BU, Lawal LO (2017). Comparative studies of phytochemical constituents of leaf , bark and root of Moringa oleifera Lam. Jewel Journal Science Reaserch. 3(1) (January 2015): 173–176.
Fachriyah E, Kusrini D, Haryanto IB, Wulandari SMB, Lestari WI, Sumariyah S (2020). Phytochemical Test, Determination of Total Phenol, Total Flavonoids and Antioxidant Activity of Ethanol Extract of Moringa Leaves (Moringa oleifera Lam). Jurnal Kimia Sains Dan Aplikasi. 23(8): 290–294. https://doi.org/10.14710/jksa.23.8.290-294
Herningtyas EH, Ng NS (2019). Prevalence and distribution of metabolic syndrome and its components among provinces and ethnic groups in Indonesia. BMC Public Health. 19(1): 377. https://doi.org/10.1186/s12889-019-6711-7
Irfan HM, Khan NAK, Asmawi MZ (2020). Moringa oleifera Lam. leaf extracts reverse metabolic syndrome in Sprague Dawley rats fed high-fructose high fat diet for 60-days. Archives of Physiology and Biochemistry. 0(0): 1–7. https://doi.org/10.1080/13813455.2020.1762661
Laoung-On J, Saenphet K, Jaikang C, Sudwan P (2021). Effect of moringa oleifera lam. Leaf tea on sexual behavior and reproductive function in male rats. Plants. 10(10): 1–17. https://doi.org/10.3390/plants10102019
Leisegang K, Henkel R, Agarwal A (2019). Obesity and metabolic syndrome associated with systemic inflammation and the impact on the male reproductive system. American Journal of Reproductive Immunology. 82(5): 1–14. https://doi.org/10.1111/aji.13178
Maslov LN, Naryzhnaya NV, Boshchenko, AA, Popov SV, Ivanov VV, Oeltgen PR (2019). Is oxidative stress of adipocytes a cause or a consequence of the metabolic syndrome? Journal of Clinical and Translational Endocrinology. 15(May 2018): 1–5. https://doi.org/10.1016/j.jcte.2018.11.001
Mohamed MA, Ahmed MA, El Sayed RA (2019). Molecular effects of Moringa leaf extract on insulin resistance and reproductive function in hyperinsulinemic male rats. Journal of Diabetes & Metabolic Disorders. 18(2): 487–494. https://doi.org/10.1007/s40200-019-00454-7
Muhammad N, Ibrahim KG, Ndhlala AR, Erlwanger KH (2020). Moringa oleifera Lam. prevents the development of high fructose diet-induced fatty liver. South African Journal of Botany, 129, 32–39. https://doi.org/10.1016/j.sajb.2018.12.003
Nagy, Fisi, Frank, Kátai, Nagy, Miseta (2019). Hyperglycemia-Induced Aberrant Cell Proliferation; A Metabolic Challenge Mediated by Protein O-GlcNAc Modification. Cells. 8(9): 999. https://doi.org/10.3390/cells8090999
Owolabi JO, OP (2014). Histological evaluation of the effects of Moringa leaf extract treatment on vital organs of murine models. Merit Research Journal of Medicine and Medical Sciences. 2(10): 245–257. Retrieved from http://www.meritresearchjournals.org/er/index.htm
Saklayen MG (2018). The Global Epidemic of the Metabolic Syndrome. Current Hypertension Reports. 20(2): 12. https://doi.org/10.1007/s11906-018-0812-z
Samson SL, Garber AJ (2014). Metabolic Syndrome. Endocrinology and Metabolism Clinics of North America. 43(1): 1–23. https://doi.org/10.1016/j.ecl.2013.09.009
Shamsi-Gamchi N, Razi M , Behfar M (2021). Cross-link between mitochondrial-dependent apoptosis and cell cycle checkpoint proteins after experimental torsion and detorsion in rats. Gene. 795 (April): 145793. https://doi.org/10.1016/j.gene.2021.145793
Sulistyorini R, Sarjadi, Johan A, Djamiatun K (2015). Pengaruh Ekstrak Etanol Daun Kelor (Moringa oleifera) pada Ekspresi Insulin dan Insulitis Tikus Diabetes Melitus. MKB. 47(2): 69–76. https://doi.org/http://dx.doi.org/10.15395/mkb.v47n2.456
Tolouei Azar J, Habibi Maleki A, Moshari S, Razi M (2020). The effect of different types of exercise training on diet-induced obesity in rats, cross-talk between cell cycle proteins and apoptosis in testis. Gene. 754 (2020): 144850 https://doi.org/10.1016/j.gene.2020.144850
Tshabalala T, Ndhlala AR, Ncube B, Abdelgadir HA, Van Staden J (2020). Potential substitution of the root with the leaf in the use of Moringa oleifera for antimicrobial, antidiabetic and antioxidant properties. South African Journal of Botany. 129: 106–112. https://doi.org/10.1016/j.sajb.2019.01.029
Widiastini LP, Karuniadi IGAM, Karmaya, INM, Widhiantara IG (2022). The Potential of A Balinese Traditional Medicine Kelor Leaves (Moringa oleifera) For Male Infertility Treatment : A Mini Review. Indian Journal of Forensic Medicine & Toxicology. 16(1): 734–742. https://doi.org/10.37506/ijfmt.v16i1.17579
Zamir-Nasta T, Razi M, Shapour H, Malekinejad H (2018). Roles of p21, p53, cyclin D1, CDK-4, estrogen receptor α in aflatoxin B1-induced cytotoxicity in testicular tissue of mice. Environmental Toxicology. 33(4): 385–395. https://doi.org/10.1002/tox.22524
Zhao L, Pang A (2020). Effects of Metabolic Syndrome on Semen Quality and Circulating Sex Hormones: A Systematic Review and Meta-Analysis. Frontiers in Endocrinology. 11(August): 1–13. https://doi.org/10.3389/fendo.2020.00428
