Comparison of β-Hydroxylase Enzyme 11 Serum in Obese, Overweight, and Normoweight Young Men


  • Jenny Novina Sitepu Faculty of Medicine, HKBP Nommensen Medan University, Medan
  • Mutiara Indah Sari Faculty of Medicine, HKBP Nommensen Medan University, Medan
  • Gino Tann Faculty of Medicine, HKBP Nommensen Medan University, Medan


Background: Previous studies showed that cardiovascular risk factor was increased in obese and overweight subjects. Obesity and cardiovascular risk factor are associated with hypothalamic-pituitary-adrenal (HPA) axis hyperactivity that causes hypercortisolism, cortisol level is associated with cardiovascular risk factor on obesity. 11 β-hydroxylase is an enzyme that involved in cortisol synthesis. The aim of this study was to investigate 11 β-Hydroxylase concentration in obesity, overweight, and normal weight young men.

Subjects and Method: This was analytic-observational study using cross-sectional design. The study was conducted at HKBP Nommensen University, Medan. The study subjects included by 76 young men aged 18-28 years old, consisting of 25 obese subjects, 25 overweight, and 25 normoweight. The concentration of 11 β-Hydroxylase was evaluated in blood sample after 10 hours fasting. The data was analyzed bivariately.

Results: Mean of 11 β-Hydroxylase concentration was 52.76 ± 44.27 in obese subjects, 70.16 ± 46.83 in overweight subjects, and 43.42 ± 27.75 in normoweight subjects. The 11 β-Hydroxylase concentration in overweight subjects was statistically higher than normoweight subjects (p = 0.007), but the 11 β-Hydroxylase concentration on obese subjects statistically was not different from normoweight subjects (p = 0.362).

Conclusion: The 11 β-Hydroxylase concentration on overweight subject is higher than normoweight subject. There is no difference of 11 β-Hydroxylase concentration on obese and normoweight subject. Mitochondrial stress and mitochondrial failure mechanism on overweight and obesity merit further investigation.

Keywords: 11 β-Hydroxylase, cortisol, obesity, overweight

Correspondence: Jenny Novina Sitepu. Faculty of Medicine, HKBP Nommensen Medan University, Medan.

Indonesian Journal of Medicine (2016), 1(1): 71-75


Anagnostis P, Athyros VG, Tziomalos KA, Karagiannis, Mikhailids DP (2009). The pa­thogenetic role of cortisol in the metabolic syndrome: a hypo­thesis. J. Clin Endocrinol Metab. 94 (8): 2692-2701.

Andrabi SMS, Bhat MH, Andrabi SRS, Ka­mi­li MMA, A Imran, Nisar I (2013). Prevalence of metabolic syndrome in 8-18 year old school going children of srinagar city of kashmir india. Indian Journal of Endocrinology and Meta­bolism, 17: 95-100.

Barret K, Brooks H, Boitano S, Barman S (2010). Ganong's review of medical phy­siology 23th edition. USA: The McGraw Hill Companies, 337-90.

Humayun A, Shah AS, Alam S, Hussein H (2009). Relationship of body mass in­dex and dyslipidemia in different age groups of male and female popu­lation of peshawar. J Ayub Med Coll Ab­bottabad 2009; 21 (2).

Inoue S, Zimmet P, Caterson I, Chunming C, Ikeda Y, Khalid AK, Kim YS, Basset J (2000). The asia pacific perspec­tive: Redefining obesity and its treat­ment.WHO/WPR / Iaso/ IOTF [avai­l­able from: http://www.wpro.­ nutrition/documents/docs/Redefiningobesity.pdf?ua=1]. accessed 9 April 2014.

Juonala M, Magnussen CG, Berenson GS, Venn A, Burns TL, Sabin MA, Rai­ta­kari OT (2011). Childhood adi­posity, adult adiposity, and cardiovas­cular risk­factors. N Engl J Med, 365: 1876-85.

Kaminski RM, Rogawski MA (2011). 11 β-hydroxylase inhibitors as protect a­gaints seizures in mice by increasing en­do­ge­nous neuroactive steroid syn­thesis. Neuropharmacology, 6(1):133-137.

Kargi AY, Iacobellis G (2014). Adipose tis­sue and adrenal glands: novel patho­physiological mechanism and clinical applications. International Journal of Endocrinology Volume 614 074 2014. ID.

McPhee SJ, Papadakis MA, Rabow MW. (2011). Current medical diagnosis and treat­ment, 15th edition. USA: Mc­Graw Hill Companies, 1202-4.

Miller WL, Auchus RJ (2011). The mole­cular biology, biochemistry, and phy­sio­logy of human steroidogenesis and its disorders. Endocrine reviews 32: 81-151, 2011.

Obregon MJ (2010). Maternal obesity re­sults in offspring prone to metabolic syndrome. Journal of Endocrinology 151 (8): 3475-3476.

Prasad R, Kowalczyk JC, Meimaridou E, Storr HL, Metherell LA. (2014). Oxi­dative stress and adrenocortical insuf­ficiency. Journal of Endocri­nology. 221, R63-R73.

Prodam F, Ricotti R, Agarla V, Parlamento S, G Genoni, Balossini C, S Bellone (2013). High end normal adrenocor­ticotropic hormone and cortisol levels are associated with specific cardiovas­cular risk factors in pediatric obesity: a cross sectional study. BMC Medi­cine, 11:44.

Purnell JQ, Brandon DD, Isabelle LM, Lo­riaux DL, MH Samuels. (2004). As­so­cia­tion of a 24-hour cortisol pro­duc­tion rates, cortisol binding glo­bulin, and plasma free cortisol levels with body composition, leptin levels, abd aging in adult men and women. J. Clin. Endocrinol. Metab, 89 (1): 281-287.

Ramzan M, Ali I, Ramzan F, Ramzan F, Ram­zan MH (2011). Waist circum­ference and lipid profile Among primary school children. JPMI, 25(3): 222-226.

Basic Health Research (Riskesdas) (2013). Agency for Health Research and Development Ministry of Health of the Republic of Indonesia Year 2013 Jakarta: Ministry of Health of the Republic of Indonesia.

Rizk NM, Yousef M (2012). Association of lipid profile and waist circumference as cardiovascular risk factors for over­weight and obesity Among school chil­dren in qatar. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 2012: 5425-432.

Russel M, Bredella M, Tsai P, Miller KK, Klibanski A, Misra M (2009). Rela­tive growth hormone and excess are as­so­ciated with Increased cardio­vas­cular risk markers in obese adoles­cents girls. J Clin Endocrinol Metab 94: 2864-2871.

Schmidt M, Johannesdottir SA, Lemeshow SA, Lash TL, Ulrichsen SP, Botker HE, So­ren­sen HT. (2013). Obesity in young men, and individual and combined risks of type 2 diabetes, cardiovascular morbidity and death before 55 years of age: danish 33-years follow-up study.

JS Thakur, Bisht S (2010). Comparative study of blood lipid profile of obese and non-obese sedentary college men. VSRD-TNTJ. Vol. I (1), 2010, 26-29

Tirosh A, Shai I, Afek A, Dubnov Raz G, Ayalon N, Gordon B, Rudich A. (2011). Adolescent bmi trajectory and risk of diabetes versus coronary disease. N Engl J Med 364: 1315-25.

World Health Organization. (2008). Waist Circumference and Waist-Hip Ratio; Report of a WHO Expert Consul­tation. Geneva: WHO.

World Health Organization (2011). Non­com­municable Diseases. Country Pro­files. Geneva: WHO.

World Health Organization (2013). Global Health Statistics 2013 Part III. Global Health Indicators. Geneva: WHO.