The Determination of Haemostasis Value in Relation to Potential Blood Leaf Leaves Filtrate (Excoecaria cochinchinensis L) as the External Medicine for Skin Wound of White Rat (Rattus norvegicus)

Siti Zaetun, Lalu Srigede


Background: Plants are currently used as medicine, i.e. medicinal plants. Medicinal plants con­sist of many chemical compounds, especially bioactive substances. One of them is Sambang Darah (Excoecaria cochinchinensis). Sambang Darah have so many benefits which usually applied in vulnus combutio. This study aimed to determine value of haemostasis after applied Sambang Darah leaves in white rat skins (Rattus norvegicus).

Subjects and Method: This was a true experimental study with post test only control group de­sign. This study conducted at Mata-ram University, West Nusa Tenggara, Indo-nesia, from April to October 2017. A total sam-ple of 16 whi­te rats was selected for this study. The dependent variable in this study was the value of haemostasis and the independent vari-able was filtrate of Sambang Darah leaves. The data were analyzed using Man Whitney test.

Results: The average level of bleeding time was 3.76 minutes for the control group and 2.58 mi­nu­tes for test group. The average level of clotting time were 2.20 minutes for control group and 1.36 mi­nutes test group. The number of platelet was 277,000/uL for the control group and 221,000/uL for test group. APTT level was25.06 second for the control group and there was no clot in test gro­up. PT level was 13.95 second for the control group and there was no clot in test group. TT levels was 18.2 second for the control group and there was no clot in test group. And the last, D Dimer level was 0.1 mg/l for the control group and there was no clot in test group. The statistical analysis sho­wed p ≤0.001.

Conclusion: Filtrat of sambang darah leaves can be applied as medicine for external wounds but it can not be applied in koagulasi test.

Keywords: Haemostasis value, blood leaf filtrate (Excoecaria cochinchinensis L)

Correspondence: Siti Zaetun.Program Study of Health Analyst, School of Health Polytechnic, Ministry of Health, Mataram. Jl. Prabu Rangkasari, Dasan Cermen, Sandubaya, Mataram, West Nusa Tenggara. Email: Mo-bile: 081803630063.

Indonesian Journal of Medicine (2020), 5(1): 10-16

Full Text:



Daniel (2010). Isolation and identification of flavonoid compounds in ethyl acetate faction from leaves of red betel plants (Piper crocatum Ruiz Pav). Jurnal Mulawarman Scientific, 9(1).

Oktariza S, Ma’aruf Y, Etika SB (2013). Isolation and Characterization of Flavonoids from Blood Leaves (Excoecaria chochinchinensis L). Jurnal Kimia Universitas Negeri Padang. 2(2): 22-29.

Putri P (2012). The effects of Giving Betel Leaf Extract (Piper betle L.) on the Amount of Leukocytes in the Edges of Animal Models Try Male Wistar Rats Exposed by Candida Albicans by Intracidation. Skripsi.Jember: Fakultas Kedokteran Gigi Universitas Jember.

Puzi WS, Lukmayani Y, Dasuki UA (2015). Isolation and Identification of Flavonoid Compounds from Red Betel Leaf (Piper crocatum Ruiz Pav). Bandung: Prosiding Penelitian SPeSIA Universitas Islam Bandung.

Susilawati E (2015). Potential of Talas stem sap (Colocasia esculenta (L.) Schott) in the process of stopping bleeding in skin lesions of white rat (Mus musculus). Karya Tulis Ilmiah. Mataram: Politeknik Kesehatan Mataram.

Sutopo T (2016). Test of 70% ethanol extract of Betel leaves (Piper betle L.) on bleeding time on male webster swiss mouse strain. Surakarta: Fakultas Kedokteran Universitas Muhammadiyah Surakarta.

Widyana L (2013). Acute toxicity test of Ambon banana stem (Musa paradisiacal var.sapientum) extract on white rat liver (Mus musculus) with LD50 Parameter. Skripsi. Surabaya: Fakultas Kedokteran Gigi Universitas Airlangga.

Zega SV (2013). Isolation of Flavonoids from Blood Leaves (Excoecaria chochinchinensis L). Medan: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sumatera Utara.


  • There are currently no refbacks.